Fosun Pharma COVID-19-RT-qPCR Detection Kit

Catalog No. PCSYHF03-a

The Fosun COVID-19 RT-PCR Detection Kit is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from the SARS-CoV-2 in upper and lower respiratory specimens from individuals suspected of COVID-19 by their healthcare provider.


80 mins

Amplification Time





96 Tests

Per Kit

Fosun Pharma: Triple Play Covid-19 RT-PCR Detection Kit Targeting Three Genes

The Benefits



Multiple Specimen Types, Including Nasopharyngeal Swab, Oropharyngeal Swab, and Sputum



3 Target Regions in One Test, including ORF1ab, N and E genes


Reliable Results

Internal control, positive and negative; UNG enzyme and dUTP used to reduce risk of contamination, false positive and false negative results



Detection of 96 samples
in 1 hour 20 minutes

Targeting Three Genes


3 Target Regions in One Test, including ORF1ab, N and E genes.


Internal Control: MS2

How It Works

1. Sample Collection

The Fosun Pharma COVID-19-RT-qPCR Detection Kit must be used with RNA extracted from nasopharyngeal or oropharyngeal samples. Sample collection should be conducted by qualified health care providers. The sample can be stored at 2°C to 8°C for 24 hours. Samples shall be transported at low temperature in accordance with biosafety regulations.

2. Sample Preparation & Extraction

Prepare reagent with ice box, and prepare reaction reagent according to the number of reaction samples (number of reaction samples, n = number of samples to be tested + 2 control samples + 1)

3. Real-time PCR

PCR equipment with applicable software is required to run the test: Applied Biosystems® 7500 instrument

4. Analytics & Reporting

Todos Medical offers custom solutions for data analytics and reporting.

What's Included?

Part #: PZHF01B-a
Description: dNTPs, MgCl2, Primers and Probes (for ORF1ab gene, E gene and N gene of SARS-CoV-2, and Internal Reference A)
Pack Size: 2 vial
Quantity: 672 μL

Part #: PZHF02B

Description: Taq DNA polymerase, Reverse Transcriptase, UNG enzyme
Pack Size: 2 vial
Quantity: 288 μL

Part #: PZHFQ-a

Description: RNA template
Pack Size: 2 vial
Quantity: 200 μL

Part #: PZHFY

Description: NaCl
Pack Size: 2 vial
Quantity: 200 μL


Part #: PZHF03B

Description: Lentivirus
Pack Size: 2 vial
Quantity: 240 μL



How To Use

1. Sample collection:
•Nasopharyngeal or oropharyngeal swabs
•Collection tubes or sputum containers
•Transport media

2. Nucleic acid extraction:
•The Recommended RNA Extraction Kit: (Not provided with the Kit) QIAamp®Viral RNA Mini Kit
•Catalog No.:(52904/52906)
•No. of preps: 50/250
•Supplier: QIAGEN®

3. Other reagents and consumables required but not included with the test:
•RNase/DNase free water, anhydrous ethanol
•96 well plates, pipette tips with filters, microcentrifuge tubes
•Liquid waste container, solid waste bag and container
•Double-layer latex gloves, waterproof boot covers, protective clothing, goggles, and masks with higher filtration efficiency.

4. Other instruments required but not included with the test:
•Applied Biosystems® 7500 instrument (software version #v1.4 or v1.5)
•PCR hood, centrifuge with rotor for 1.5 mL and 2 mL tubes, vortex mixer, metal bath, and pipette.

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